M.B. Enzymes GmbH

Genomic DNA

Description

High molecular weight genomic DNAs purified from blood or other nonglycogen-containing tissues to obtain intact genomic DNA with reduced turbidity. Based on modified salt precipitation procedure, DNA is recovered free of protein and RNA contaminations, on average is >100 kb in length, and has A₂₆₀/A₂₈₀ ratios between 1.7-2.0.

All donors of human genomic DNA was tested negative for HIV-1, HIV-2, HCV, HBV and syphilis (PCR, ELISA).

Storage

Store at +4°C.

Storage buffer

10 mM Tris-HCl pH 7.8, 10 mM NaCl, 1 mM EDTA.

Applications

Southern blot analysis, DNA amplification, genomic research.

Quality control

spectrophotometric control; absence of contaminating nucleases; tested for integrity in PFGE.

References

1. Sambrook, J. et al. (1989) Molecular Cloning: A laboratory manual, CSH Laboratory, NY, 9.14. 2. Blin, N., Stafford, D.W. (1976) Nucl. Acids Res. 3, 2303-2308.

Catalog #

D3.1 male

D3.2 female

Pack size

100 µg

100 µg

Genomic DNA
Description	High molecular weight genomic DNAs purified from blood or other nonglycogen-containing tissues to obtain intact genomic DNA with reduced turbidity. Based on modified salt precipitation procedure, DNA is recovered free of protein and RNA contaminations, on average is >100 kb in length, and has A₂₆₀/A₂₈₀ ratios between 1.7-2.0. All donors of human genomic DNA was tested negative for HIV-1, HIV-2, HCV, HBV and syphilis (PCR, ELISA).
Storage	Store at +4°C.
Storage buffer	10 mM Tris-HCl pH 7.8, 10 mM NaCl, 1 mM EDTA.
Applications	Southern blot analysis, DNA amplification, genomic research.
Quality control	spectrophotometric control; absence of contaminating nucleases; tested for integrity in PFGE.
References	1. Sambrook, J. et al. (1989) Molecular Cloning: A laboratory manual, CSH Laboratory, NY, 9.14. 2. Blin, N., Stafford, D.W. (1976) Nucl. Acids Res. 3, 2303-2308.
Catalog #	D3.1 male	D3.2 female
Pack size	100 µg	100 µg