Bacteriophage lambda DNA.
Description	Unmethylated cl857ind1Sam7 Bacteriophage lambda DNA (GenBank #NC_001416) is isolated from infected GM 119, an E.coli strain lacking both dam and dcm methylase activities. The DNA is linear and double-stranded with 12 bp single-stranded complementary 5´-ends (cos sites). It has a molecular weight of 31,5x106 Da and contains 48.502 bp.
Storage	Store at +4°C to -20°C. Upon storage at +4°C the DNA forms linear aggregates as well as circular structures due to the presence of cohesive termini. Heating of the DNA to75°C for 10 min followed by quick cooling will reversible restore it to its linear form.
Storage buffer	10 mM Tris-HCl pH 7.8, 10 mM NaCl, 1 mM EDTA.
Applications	used as substrate for restriction enzymes.
Quality control	spectrophotometric control, absence of contaminating ds nuclease activity, proper restriction fragments.
References	1. Sanger, F. et al. (1982) J. Mol. Biol. 162, 729. 2. Arraj, J.A., Marinus, M.G. (1983) J.Bacteriol. 153, 562.
Catalog #	D1.3
Pack size	500 µg