T4 Gene 32 Protein.
Description	T4 gp32 is a single-strand DNA binding protein with M.W. 33,5 kDa which stabilizes ss regions of DNA and RNA and destabilizes helixes. It is reported to increase the specificity of PCR reactions and to improve the yield of long PCR products. Furthermore it is described to prevent inhibition of PCR caused by the presence of blood, to improve DNA sequencing of templates with secondary structures and to drive large scale restriction enzyme digests to completion.
Reaction conditions	T4 gp32 should be added to the reaction mixture at a final conc. of 1 nM.
Storage	Store at -20°C.
Applications	PCR, DNA sequencing, in studies of ssDNA-protein interactions and to visualize ssDNA by electron microscopy.
Quality control	activity, SDS-PAGE purity, absence of endo- and exonucleases.
References	1. Cohen, H.A. et al. (1983) J. Mol. Biol. 169, 235-248. 2. Tsugita, A., Hosada, J. (1978) J. Mol. Biol. 122, 255-258. 3. Schwarz, K. et al. (1990) Nucl. Acids Res. 18, 1079.
Catalog #	E1.1	E1.2
Pack size	100 µg	250 µg