M.B. Enzymes GmbH

RT enhancer.

Description

Reverse Transcriptase Enhancer (RTE) is an additive that can greatly increase the yield and specificity of reverse transcription. RT-Enhancer destabilizes hairpin RNA structure and activates annealing of the primer to RNA. The addition of RT-Enhancer generates greater yields in case of limited amounts of RNA using standard conditions, as well as results in a substantial increase in RT specificity and size of resulted product.

Reaction conditions

use 1 unit of RT-Enhancer per 20 µl reaction.

Storage

Store at -20°C.

Applications

First strand cDNA synthesis for cloning and hybridization, RNA sequencing, RT-PCR.

Quality control

activity, SDS-PAGE purity, no contaminating endo- and exonuclease activities.

References

Catalog #

E3.3

E3.4

Pack size

500 U

1000 U

RT enhancer.
Description	Reverse Transcriptase Enhancer (RTE) is an additive that can greatly increase the yield and specificity of reverse transcription. RT-Enhancer destabilizes hairpin RNA structure and activates annealing of the primer to RNA. The addition of RT-Enhancer generates greater yields in case of limited amounts of RNA using standard conditions, as well as results in a substantial increase in RT specificity and size of resulted product.
Reaction conditions	use 1 unit of RT-Enhancer per 20 µl reaction.
Storage	Store at -20°C.
Applications	First strand cDNA synthesis for cloning and hybridization, RNA sequencing, RT-PCR.
Quality control	activity, SDS-PAGE purity, no contaminating endo- and exonuclease activities.
References
Catalog #	E3.3	E3.4
Pack size	500 U	1000 U