SP6 RNA polymerase.
SP6 RNA polymerase (EC is a DNA-dependent RNA polymerase that recognizes and initiates synthesis of RNA on dsDNA templates that carry the appropriate SP6 promoter (5´ATTTAGGTGACACTATA3´). The enzyme is a single polypeptide and is isolated from an overproducing recombinant E. coli strain.
Unit definition

One unit of the enzyme catalyzes the incorporation of 1 nmol of 3H ATP into acid-insoluble product in 60 min at 40°C.

Reaction buffers
B13 (x5)
Reaction conditions
10 U SP6 RNA polymerase, 2 µg template DNA, 40 mM Tris-HCl pH 7.5, 6 mM MgCl2, 2 mM spermidine-HCl, 10 mM DTT, 1 mM rNTPs. Additionally, inorganic pyrophosphotase can be added to a final concentration of 4 U/ml to enhance the polymerization reaction. The enzyme will incorporate 32P, 35S and 3H nucleoside triphosphates
Store at -20°C.
production of antisense RNA; RNA labeling; mRNA generation for in vitro translation.

Quality control

activity, SDS-PAGE purity, absence of RNase and DNase activities, specific performance tests.


1. Butler, E.T., Chamberlin, M.J. (1982) J. Biol. Chem. 257, 5772. 2. Melton, D.A. et al. (1984) Nucl. Acids Res. 12, 7035.

Catalog #





Pack size

2.000 U






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