E.coli RNase H.
Description	E. coli RNase H (EC 3.1.26.4) specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3'-hydroxil and 5'-phosphate terminated products. It will not degrade single-stranded nucleic acids, duplex DNA or double-stranded RNA.
Unit definition	One unit is defined as the amount of enzyme required to produce 1 nmol acid-soluble ribonucleotides from 0,5 nM radiolabeled poly(rA):poly(dT) in 20 min at 37°C.
Storage	Store at -20°C.
Applications	elimination of RNA during second strand cDNA synthesis; detection of RNA:DNA regions in dsDNA of natural origin; oligodeoxyribonucleotide-directed cleavage of RNA; removal of poly(A) sequences from mRNA in the presence of oligo(dT).
Quality control	activity, SDS-PAGE purity, absence of dsDNases, nickases and unspecific RNase activity.
References	1. Gubbler, U., Hoffman, B.J. (1983) Gene 25, 263-269. 2. Davis, R. et al. (1988) Mol. Cell Biol. 8, 4745-4755. 3. Donis-Keller, H. (1979) Nucl. Acids Res. 7, 179. 4. Goodwin, E.C., Rottman, F.M. (1992) Nucl. Acids Res. 20, 916.
Catalog #	M10.3	M10.4
Pack size	500 U	1.000 U