Ribonuclease T1.
Description	Ribonuclease T1 (RNase T1, EC 3.1.27.3 )is isolated from an overexpressing E.coli strain containing the cloned Aspergillus oryzae gene. The enzyme specifically attacks the guanine sites in ssRNA to yield 3´-GMP and oligonucleotides with a 3´-GMP group. Activity approx. 500kU/mg protein.
Unit definition	One unit produces acid soluble oligonucleotides equivalent to DA260 of 1.0 per 30 min at 37°C and pH 7.5.
Reaction conditions	At low salt concentrations (<100 mM NaCl), it cleaves ss and dsRNA as well as the RNA strand in RNA:DNA duplexes. RNase T1 is active under a wide range of reaction conditions, and it is difficult to inactivate. Removal of RNase T1 from a reaction solution generally requires treatment with proteinase K followed by multiple phenol extractions and ethanol precipitation.
Storage	Store at +4°C.
Applications	determining the level of RNA transcripts synthesized in vitro from DNA templates containing a "G-less cassette"; in conjnction with RNase A in RNase protection assay; RNA fingerprinting; removing unhybridized regions of RNA from DNA:RNA or RNA:RNA hybrids.
Quality control	activity, minimum 70% SDS-PAGE purity, no detectable DNase and protease activities.
Reference	1. Uchida, T., Egami, F. (1971) In The Enzymes, vol. IV, 205-250.
Catalog #	M13.1
Pack size	100 kU