DNase I, RNase free.
Deoxyribonuclease I (EC is isolated from bovine pancreas catalyzes the hydrolysis of dsDNA to produce 3´-hydroxyl oligonucleotides; at high concentration of the enzyme, ssDNA will also be digested. In the presence of Mg2+ DNase I produces nicks in dsDNA, while in the presence of Mn2+ the enzyme produces ds breaks. M.W. approx. 37000. Specific activity approx. 3000 U/mg protein.
Unit definition

One unit will cause an increase in absorbance of calf thymus DNA solution at a rate of 0,001 A260 units/min/ml at 25°C and pH 5.0.

Reaction conditions
50 mM Tris-HCl pH 7.5, 10 mM MnCl2 (or 10 mM MgCl2in nick translation protocols), 50 µg/ml BSA, 1 µg DNA, 0,1-2U of the enzyme (degradative use) or 20 mU(nick translation) in a 100µl reaction volume. Incubate at 37°C for 60 min. Stop reaction by adding EDTA to 25 mM (end conc.).
Store at -20°C.
nick translation; DNase footprinting, preparation of DNA-free RNA (RT-PCR, in vitro transcription/translation systems), cloning random DNA fragments by catalyzing ds cleavage of DNA in the presence of Mn2+.

Quality control

activity, SDS-PAGE purity, no contaminating ribonuclease activity.


1. Kunitz, M. (1950) J. gen. Physiol. 33, 363. 2. Moor, S. (1981) In The Enzymes, vol 14A, 281-298. 3. Campbel, V.W., Jackson, D.A. (1980) J. Biol. Chem. 255, 3726-3735.

Catalog #





Pack size

2.500 U

10.000 U





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