M.B. Enzymes GmbH

b-Agarase I.

Description

b-Agarase I (EC 3.2.1.81) is isolated from an overexpressing E.coli strain containing the cloned Pseudomonas atlantica gene. The enzyme specifically digest the agarose polysaccharide core made up of repeating 1,3-linked b-D-galactopyranose and 1,4-linked 3,6-anhydro-a-L-galactopyranose into neoagaro-oligosaccharides. M.W. approx. 32700. Specific activity approx. 25 U/mg protein.

Unit definition

One unit will digest 100 µl of molten LMP agarose in 30 min at 40°C to completion.

Reaction conditions

The enzyme works equally well in different buffers (0.5xTBE, 1xTAE, 1xTPE or 1xBis-Tris); other recommendable buffer formulations include 1 M NaAc pH 5.0 (x10) or 100 mM Bis-Tris-HCl pH 6.5, 10 mM EDTA (x10).

Storage

Store at -20°C.

Applications

used for purification of nucleic acids from low melting point (LMP) agaroses.

Quality control

activity, SDS-PAG purity, no contaminating nuclease and phosphatase activity.

References

1. Yaphe, W. (1957) Can. J. Microbiol. 3, 987-993. 2. Schedl, A. et al. (1993) Nature 362, 258. 3. Steck, T.R. (1994) BioTechniques 17, 676.

Catalog #

M16.1

M16.3

Pack size

100 U

500 U

b-Agarase I.
Description	b-Agarase I (EC 3.2.1.81) is isolated from an overexpressing E.coli strain containing the cloned Pseudomonas atlantica gene. The enzyme specifically digest the agarose polysaccharide core made up of repeating 1,3-linked b-D-galactopyranose and 1,4-linked 3,6-anhydro-a-L-galactopyranose into neoagaro-oligosaccharides. M.W. approx. 32700. Specific activity approx. 25 U/mg protein.
Unit definition	One unit will digest 100 µl of molten LMP agarose in 30 min at 40°C to completion.
Reaction conditions	The enzyme works equally well in different buffers (0.5xTBE, 1xTAE, 1xTPE or 1xBis-Tris); other recommendable buffer formulations include 1 M NaAc pH 5.0 (x10) or 100 mM Bis-Tris-HCl pH 6.5, 10 mM EDTA (x10).
Storage	Store at -20°C.
Applications	used for purification of nucleic acids from low melting point (LMP) agaroses.
Quality control	activity, SDS-PAG purity, no contaminating nuclease and phosphatase activity.
References	1. Yaphe, W. (1957) Can. J. Microbiol. 3, 987-993. 2. Schedl, A. et al. (1993) Nature 362, 258. 3. Steck, T.R. (1994) BioTechniques 17, 676.
Catalog #	M16.1	M16.3
Pack size	100 U	500 U