M.B. Enzymes GmbH

Nucleotides -2´-Deoxyribonucleoside-5´-triphosphate solutions. Biotin-11-dUTP.

Description

High quality solutions of dATP, dGTP, dCTP and dTTP are available either as individual nucleotides or as a mixture of all 4 nucleotides for use in DNA synthesis. Solutions are available at 2 mM (x10 concentrated), 20 mM (x100 concentrated) and 100 mM (x500 concentrated). 2 mM and 100 mM solutions of dUTP as well as biotin-11-dUTP are also available for synthesis of uracil-containing DNA and non-radioactive labeling.

dNTP solutions are made with sterile deionized water and adjusted to pH 7.0 with NaOH.

dATP C₁₀H₁₆N₅O₁₂P₃ Mr 491,2

dGTP C₁₀H₁₆N₅O₁₃P₃ Mr 507,2

dCTP C₉H₁₆N₃O₁₃P₃ Mr 467,1

dTTP C₁₀H₁₇N₂O₁₄P₃ Mr 482,1

dUTP C₉H₁₅N₂O₁₄P₃ Mr 468,1

biotin-11-dUTP C₂₈H₄₅N₆O₁₇P₃S Mr 862,7

Storage

Store preferably at -20°C. dNTPs are also stable at room temperature for months without loss of quality.

Applications

DNA synthesis and non-radioactive labeling.

Quality control

all dNTP solutions meet spectrophotometric criteria and are functionally tested in PCR.

References

1. Hoard, D.E., Ott, D.G. (1965) J. Am. Chem. Soc. 87, 1785-1788. 2. Yoshikawa, M. et al. (1967) Tetrahedron Letters 5065.

Premixed dNTP solutions. Supplied in a volume of 250 µl

Cat.# N1.2 20 mM (5µmol) of each dATP, dGTP, dCTP and dTTP

Cat.# N2.2 2 mM (0,5µmol) of each dATP, dGTP, dCTP and dTTP

Cat.# N9.1 dNTP set 4x 100 mM (25µmol) of each dNTP (dATP, dGTP, dCTP and dTTP)

Individual dNTP solutions. Supplied in a volume of 250 µl

Cat.# N3.2 100 mM (25µmol) dATP

Cat.# N4.2 100 mM (25µmol) dGTP

Cat.# N5.2 100 mM (25µmol) dCTP

Cat.# N6.2 100 mM (25µmol) dTTP

Cat.# N7.2 100 mM (25µmol) dUTP

Cat.# N8.2 100 mM (25µmol) biotin-11-dUTP

Nucleotides -2´-Deoxyribonucleoside-5´-triphosphate solutions. Biotin-11-dUTP.
Description	High quality solutions of dATP, dGTP, dCTP and dTTP are available either as individual nucleotides or as a mixture of all 4 nucleotides for use in DNA synthesis. Solutions are available at 2 mM (x10 concentrated), 20 mM (x100 concentrated) and 100 mM (x500 concentrated). 2 mM and 100 mM solutions of dUTP as well as biotin-11-dUTP are also available for synthesis of uracil-containing DNA and non-radioactive labeling. dNTP solutions are made with sterile deionized water and adjusted to pH 7.0 with NaOH. dATP C₁₀H₁₆N₅O₁₂P₃ Mr 491,2 dGTP C₁₀H₁₆N₅O₁₃P₃ Mr 507,2 dCTP C₉H₁₆N₃O₁₃P₃ Mr 467,1 dTTP C₁₀H₁₇N₂O₁₄P₃ Mr 482,1 dUTP C₉H₁₅N₂O₁₄P₃ Mr 468,1 biotin-11-dUTP C₂₈H₄₅N₆O₁₇P₃S Mr 862,7
Storage	Store preferably at -20°C. dNTPs are also stable at room temperature for months without loss of quality.
Applications	DNA synthesis and non-radioactive labeling.
Quality control	all dNTP solutions meet spectrophotometric criteria and are functionally tested in PCR.
References	1. Hoard, D.E., Ott, D.G. (1965) J. Am. Chem. Soc. 87, 1785-1788. 2. Yoshikawa, M. et al. (1967) Tetrahedron Letters 5065.
	Premixed dNTP solutions. Supplied in a volume of 250 µl Cat.# N1.2 20 mM (5µmol) of each dATP, dGTP, dCTP and dTTP Cat.# N2.2 2 mM (0,5µmol) of each dATP, dGTP, dCTP and dTTP Cat.# N9.1 dNTP set 4x 100 mM (25µmol) of each dNTP (dATP, dGTP, dCTP and dTTP) Individual dNTP solutions. Supplied in a volume of 250 µl Cat.# N3.2 100 mM (25µmol) dATP Cat.# N4.2 100 mM (25µmol) dGTP Cat.# N5.2 100 mM (25µmol) dCTP Cat.# N6.2 100 mM (25µmol) dTTP Cat.# N7.2 100 mM (25µmol) dUTP Cat.# N8.2 100 mM (25µmol) biotin-11-dUTP