Carboxypeptidase B - Protaminase.
Description	Carboxypeptidase B (Protaminase; Peptidyl-L-lysine(L-arginine) hydrolase, EC 3.4.17.2.) from porcine pancreas. M.W. approx. 34500. Activity approx.175-250 units per mg protein. Two grades of purity are available: P2.1. Lyophilized powder containing 50% protein. P2.2. Lyophilized powder containing 75% protein.
Unit definition	One unit hydrolyzes 1.0 mmole of hippuriyl-L-arginine per min at pH 7.65 at 25°C.
Storage	Store at -20°C.
Applications	used for selective cleavage of proteins for amino acid sequence determination or peptide mapping.
Quality control	Affinity purified. Lyophilized powder containing approx. 50-75% protein; balance primarily borat buffer salts, pH 7.5. Contains <1% carboxypeptidase A (hippuriyl-L-phenilalanin), no detectable trypsin (BAPNA) and chymotrypsin (Z-Ala-Ala-Leu-pNA).
References	Akama, K., Tobita, T. (1987) Anal. Sci. 3, 167.
Catalog #	P2.1	P2.2	P2.1	P2.2
Pack size	10 mg	100 mg	10 mg	100 mg