Human fetal RNA
Description	Total RNA is purified from fetal tissues of human origine using modified guanidium thiocyanate purification method. During preparation, all tissues are rapidly minced and frozen in liquid nitrogen to prevent degradation by endogenous nucleases. All tissues used for RNA isolation was tested negative for HIV-1, HIV-2, HCV, HBV and syphilis (PCR or ELISA).
Storage	All preparations are provided in ethanol suspension containing 0.08 M sodium acetate. Store at -70°C.
Applications	cDNA synthesis; RT-PCR, Northern blot and primer extension analysis, RNase protection assay.
Quality control	spectrophotometric control, absence of contaminating ss and ds nuclease activity, high molecular weight DNA and proteins; each preparation contains intact 28S and 18S ribosomal RNA when electrophoresed on a denaturing agarose gel
References	1. Chirgwin, J.M. et al. (1979) Biochemistry 18, 5294-5299. 2. Chomczynski, P., Sacchi, N. (1987) Anal. Biochem. 162, 156-159.
Catalog #	R1
Pack size	100 µg