Lambda DNA BstE II digest - Size range 117- 14.140 bp.
Description	The BstE II digest of lambda DNA (cl857 ind 1 Sam7) yields 14 fragments ranging in size from 117 bp to 8.454 bp. However, the cohesive ends of two fragments (8453 bp and 5687 bp) with cos sites may anneal to and form the additional band of 14.140 bp. Heating of the DNA to 75°C for 10 min followed by quick cooling will reversible restore this band. When end labeled using fill-in reaction, lambda BstE II digest is suitable for evaluate the size of dsDNA in experiments based on chemiluminiscent and radioactive DNA detection.
Storage	Store at +4° to -20°C.
Storage buffer	10 mM Tris-HCl pH 7.8, 10 mM NaCl, 1 mM EDTA.
Applications	used in gel electrophoresis to evaluate the size of dsDNA fragments.
Quality control	spectrophotometric control, absence of contaminating ds nuclease activity, proper restriction fragments.
References	1. Sanger, F. et al. (1982) J. Mol. Biol. 162, 729. 2. Arraj, J.A., Marinus, M.G. (1983) J.Bacteriol. 153, 562.
Catalog #	S1.1	S1.2		S1.3	S1.4
Pack size	50 µg ready-to-use	500 µg ready-to-use		50 µg water sol. +6xLoading dye	500 µg water sol +6xLoading dye