100 bp DNA Step Ladder - Size range 100-1000 bp.
The 100 bp DNA step ladder consists of 10 blunt-ended DNA fragments ranging from 100 to 1.000 bp in exactly 100 bp increments. The 500 bp and 1 kb bands are present at an increased intensity to simplify size assigment. The marker can be optimal separated and analyzed in a 1,7% agarose gel stained with ethidium bromide. Load 0,5-1 µg per lane (5-10 µl of the ready-to-use marker).The marker can be internal labeled by nick translation protocol or end-labeled using TdT and T4 DNA polymerase or in phosphate exchange reaction using T4 PNK.
The 6xloading dye (40% Sucrose, 0,1% bromophenol blue, 0,1% xylene cyanol FF, 0,4% orange G) supplied with this marker is used for loading DNA samples into gel wells and tracking migration during electrophoresis. The xylene cyanol migrates with bands of approximately 4 kb, bromophenol blue with bands of approximately 300 bp and orange G with bands of approximately 50 bp in 0,5-1,4% agarose gels using 0,5xTBE as buffer.

Store at +4° to -20°C.

Storage buffer
10 mM Tris-HCl pH 7.8, 10 mM NaCl, 1 mM EDTA.
used in gel electrophoresis to evaluate the size of dsDNA fragments.

Quality control

spectrophotometric control, absence of contaminating nucleases, accurate sizing.

Catalog #





Pack size

50 µg
500 µg
50 µg water sol.
+6xLoading dye
500 µg water sol
+6xLoading dye


Copyright © 2002 M.B. Enzymes GmbH