100 bp DNA Step Ladder - Size range 100-1000 bp.
Description	The 100 bp DNA step ladder consists of 10 blunt-ended DNA fragments ranging from 100 to 1.000 bp in exactly 100 bp increments. The 500 bp and 1 kb bands are present at an increased intensity to simplify size assigment. The marker can be optimal separated and analyzed in a 1,7% agarose gel stained with ethidium bromide. Load 0,5-1 µg per lane (5-10 µl of the ready-to-use marker).The marker can be internal labeled by nick translation protocol or end-labeled using TdT and T4 DNA polymerase or in phosphate exchange reaction using T4 PNK. The 6xloading dye (40% Sucrose, 0,1% bromophenol blue, 0,1% xylene cyanol FF, 0,4% orange G) supplied with this marker is used for loading DNA samples into gel wells and tracking migration during electrophoresis. The xylene cyanol migrates with bands of approximately 4 kb, bromophenol blue with bands of approximately 300 bp and orange G with bands of approximately 50 bp in 0,5-1,4% agarose gels using 0,5xTBE as buffer.
Storage	Store at +4° to -20°C.
Storage buffer	10 mM Tris-HCl pH 7.8, 10 mM NaCl, 1 mM EDTA.
Applications	used in gel electrophoresis to evaluate the size of dsDNA fragments.
Quality control	spectrophotometric control, absence of contaminating nucleases, accurate sizing.
Catalog #	S2.1	S2.2		S2.3	S2.4
Pack size	50 µg ready-to-use	500 µg ready-to-use		50 µg water sol. +6xLoading dye	500 µg water sol +6xLoading dye