M.B. Enzymes GmbH

1 kb DNA Step Ladder - Size range 250-10.000 bp.

Description

The 1 kb DNA ladder consists of 13 blunt-ended DNA fragments ranging in size from 250 bp to 10 kb. The 1 kb and 3 kb bands are present at an increased intensity to simplify size assigment. The marker can be internal labeled by nick translation protocol or end-labeled using TdT and T4 DNA polymerase or in phosphate exchange reaction using T4 PNK.

The marker can be optimal separated and analyzed in a 0,8-1% agarose gel stained with ethidium bromide. Load 0,5-1 µg per lane (5-10 µl of the ready-to-use marker). The 6xloading dye (40% Sucrose, 0,1% bromophenol blue, 0,1% xylene cyanol FF) supplied with this marker is used for loading DNA samples into gel wells and tracking migration during electrophoresis. The xylene cyanol migrates with bands of approximately 4 kb and bromophenol blue with bands of approximately 300 bp in 0,5-1,4% agarose gels in 0,5xTBE.

Storage

Store at +4° to -20°C. Avoid exposure to multiple freeze-thaw cycles.

Storage buffer

10 mM Tris-HCl pH 7.8, 10 mM NaCl, 1 mM EDTA.

Applications

qualified as size marker for dsDNA in gel electrophoresis experiments.

Quality control

spectrophotometric control, absence of contaminating nucleases, accurate sizing.

Catalog #

S3.1

S3.2

S3.3

S3.4

Pack size

50 µg

ready-to-use

500 µg

ready-to-use

50 µg water sol.

+6xLoading dye

500 µg water sol

+6xLoading dye

1 kb DNA Step Ladder - Size range 250-10.000 bp.
Description	The 1 kb DNA ladder consists of 13 blunt-ended DNA fragments ranging in size from 250 bp to 10 kb. The 1 kb and 3 kb bands are present at an increased intensity to simplify size assigment. The marker can be internal labeled by nick translation protocol or end-labeled using TdT and T4 DNA polymerase or in phosphate exchange reaction using T4 PNK. The marker can be optimal separated and analyzed in a 0,8-1% agarose gel stained with ethidium bromide. Load 0,5-1 µg per lane (5-10 µl of the ready-to-use marker). The 6xloading dye (40% Sucrose, 0,1% bromophenol blue, 0,1% xylene cyanol FF) supplied with this marker is used for loading DNA samples into gel wells and tracking migration during electrophoresis. The xylene cyanol migrates with bands of approximately 4 kb and bromophenol blue with bands of approximately 300 bp in 0,5-1,4% agarose gels in 0,5xTBE.
Storage	Store at +4° to -20°C. Avoid exposure to multiple freeze-thaw cycles.
Storage buffer	10 mM Tris-HCl pH 7.8, 10 mM NaCl, 1 mM EDTA.
Applications	qualified as size marker for dsDNA in gel electrophoresis experiments.
Quality control	spectrophotometric control, absence of contaminating nucleases, accurate sizing.
Catalog #	S3.1	S3.2	S3.3	S3.4
Pack size	50 µg ready-to-use	500 µg ready-to-use	50 µg water sol. +6xLoading dye	500 µg water sol +6xLoading dye