1 kb DNA Step Ladder - Size range 250-10.000 bp.
The 1 kb DNA ladder consists of 13 blunt-ended DNA fragments ranging in size from 250 bp to 10 kb. The 1 kb and 3 kb bands are present at an increased intensity to simplify size assigment. The marker can be internal labeled by nick translation protocol or end-labeled using TdT and T4 DNA polymerase or in phosphate exchange reaction using T4 PNK.
The marker can be optimal separated and analyzed in a 0,8-1% agarose gel stained with ethidium bromide. Load 0,5-1 µg per lane (5-10 µl of the ready-to-use marker). The 6xloading dye (40% Sucrose, 0,1% bromophenol blue, 0,1% xylene cyanol FF) supplied with this marker is used for loading DNA samples into gel wells and tracking migration during electrophoresis. The xylene cyanol migrates with bands of approximately 4 kb and bromophenol blue with bands of approximately 300 bp in 0,5-1,4% agarose gels in 0,5xTBE.

Store at +4° to -20°C. Avoid exposure to multiple freeze-thaw cycles.

Storage buffer
10 mM Tris-HCl pH 7.8, 10 mM NaCl, 1 mM EDTA.
qualified as size marker for dsDNA in gel electrophoresis experiments.

Quality control

spectrophotometric control, absence of contaminating nucleases, accurate sizing.

Catalog #





Pack size

50 µg
500 µg
50 µg water sol.
+6xLoading dye
500 µg water sol
+6xLoading dye


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