Thermonuclease - Thermostable structure-specific endonuclease.
Description	Thermonuclease is a thermostable endonuclease that recognizes and cleaves the junctions between single- and double-stranded DNA regions in partially duplexed DNA molecules. The DNA structure consisted of partially double-stranded hairpin regions interspersed with single-stranded regions is formed as a result of denaturation and subsequent cooling of double stranded DNA to an intermediate temperature.
Unit definition	One unit will digests 3.7 pmol of 33 nt oligonucleotide substrate, which folds to create a 12-bp stem with a 3-nt loop, in 1 min at 55°C.
Reaction buffer	B23 (x10): 100 mM Tris-HCl, 20 mM MnCl2, 1% Triton X-100 for 0.1 - 1.0 kb In case of longer fragments (0,5-2 kbp), MgCl2 should be added to the reaction mixture at a final conc. of 10 mM. Reaction can be performed also in the buffers used for thermostable DNA polymerases.
Storage	Store at -20°C.
Applications	CFLP (Cleavage Fragment Length Polymorphism).
Quality control	activity, SDS-PAGE purity.
References	1. Lyamichev, V. et al. (1993) Science 260, 778-783. 2. Brow, M.A. et al. (1996) J. Clin. Microbiol. 34, 3129-37.
Catalog #	T14.3	T14.4
Pack size	500 U	1000 U