M.B. Enzymes GmbH

Tth RNase H.

Description

Tth RNase H (EC 3.1.26.4) specifically degrades only RNA in an DNA/RNA hybrid, without affecting DNA or unhybridized RNA. The enzyme will not degrade ss nucleic acids, dsDNA or dsRNA molecules.The optimal temperature is 65°C. However, the enzyme works well at elevated temperatures up to 95°C.

Unit definition

1 unit results in the acid-solubilization of 1 nmol of ³H radiolabelled poly(dT):poly(A) in 20 min at 45°C under standard reaction conditions.

Reaction buffer

#B25 (10x): 200 mM HEPES-KOH pH 7.8, 500 mM KCl, 100 mM MgCl₂, 10 mM DTT.

Reaction conditions

Storage

Store at -20°C.

Applications

high stringency hybrid selection; isotherm probe amplification; transcription-based amplification methods; high stringency mapping of mRNA structures; amplifications where specific removal of the RNA in a DNA:RNA hybrid is required.

Quality control

activity, absence of contaminating DNA exo- and endonuclease activity.

References

1. Duck, P. et al. (1990) Biotechniques 9, 142. 2. Bekkaoui, F. et al. (1996) Biotechniques 20, 240. 3. Guatelli, J.C. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1874.

Catalog #

T15.3

T15.4

Pack size

500 U

1000 U

Tth RNase H.
Description	Tth RNase H (EC 3.1.26.4) specifically degrades only RNA in an DNA/RNA hybrid, without affecting DNA or unhybridized RNA. The enzyme will not degrade ss nucleic acids, dsDNA or dsRNA molecules.The optimal temperature is 65°C. However, the enzyme works well at elevated temperatures up to 95°C.
Unit definition	1 unit results in the acid-solubilization of 1 nmol of ³H radiolabelled poly(dT):poly(A) in 20 min at 45°C under standard reaction conditions.
Reaction buffer	#B25 (10x): 200 mM HEPES-KOH pH 7.8, 500 mM KCl, 100 mM MgCl₂, 10 mM DTT.
Reaction conditions
Storage	Store at -20°C.
Applications	high stringency hybrid selection; isotherm probe amplification; transcription-based amplification methods; high stringency mapping of mRNA structures; amplifications where specific removal of the RNA in a DNA:RNA hybrid is required.
Quality control	activity, absence of contaminating DNA exo- and endonuclease activity.
References	1. Duck, P. et al. (1990) Biotechniques 9, 142. 2. Bekkaoui, F. et al. (1996) Biotechniques 20, 240. 3. Guatelli, J.C. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 1874.
Catalog #	T15.3	T15.4
Pack size	500 U	1000 U