ThermolaseHF™ (EC enables amplification of the DNA with higher fidelity in comparison with Taq DNA polymerase. The mix combines DNA polymerases with different activities and properties to achieve more accurate amplification of the DNA templates. The enzyme mix permits efficient amplification of up to 10 kb from lambda DNA and up to 5 kb from genomic DNA.
Unit definition

One unit of the enzyme mix will incorporate 10 nmol of dNTPs into an acid-insoluble form in 30 min at 72°C under standard assay conditions using a DNA template.

Reaction buffer
B21 (x10, with Mg2+ )
Store at -20°C.
high-fidelity PCR.

Quality control

functionally tested in PCR.


1. Lundberg, K.S. et al. (1991) Gene, 108, 1-6. 2. Cheng, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 5695-5699.

Catalog #





Pack size

500 U

1000 U





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