ThermolaseRed™-Thermolase™ DNA polymerase supplied with red dye in storage buffer.
Description	ThermolaseRed™ (EC 2.7.7.7) is formulated with a red dye for better visualization and loading of PCR products onto agarose gels. For further specifications, please see Thermolase™.
Unit definition	One unit of the enzyme will incorporate 10 nmol of dNTPs into an acid-insoluble form in 30 min at 72°C under standard assay conditions using a DNA template.
Reaction buffer	B1 (x10, with Mg2+ ): 670 mM Tris-HCl, pH 8.8, 166 mM (NH4)2SO4, 15 mM MgCl2, 0.1% Tween 20.
Storage	Store at -20°C.
Applications	PCR, mutation analysis (TaqMan™, PAMSA), site-directed mutagenesis, DNA labeling, 3´ A-tailing of blunt ends; primer extension; sequencing.
Quality control	activity, SDS-PAGE purity, absence of endo- and exonucleases, specific performance tests.
References
Catalog #	T3.4	T3.5
Pack size	1000 U	2500 U