ThermolaseRed™-Thermolase™ DNA polymerase supplied with red dye in storage buffer.
ThermolaseRed™ (EC is formulated with a red dye for better visualization and loading of PCR products onto agarose gels. For further specifications, please see Thermolase™.
Unit definition

One unit of the enzyme will incorporate 10 nmol of dNTPs into an acid-insoluble form in 30 min at 72°C under standard assay conditions using a DNA template.

Reaction buffer
B1 (x10, with Mg2+ ): 670 mM Tris-HCl, pH 8.8, 166 mM (NH4)2SO4, 15 mM MgCl2, 0.1% Tween 20.
Store at -20°C.
PCR, mutation analysis (TaqMan™, PAMSA), site-directed mutagenesis, DNA labeling, 3´ A-tailing of blunt ends; primer extension; sequencing.

Quality control

activity, SDS-PAGE purity, absence of endo- and exonucleases, specific performance tests.



Catalog #





Pack size

1000 U

2500 U





Copyright © 2002 M.B. Enzymes GmbH