ThermolaseLR™ (EC enables consistent amplification of long DNA sequence. The mix combines DNA polymerases with different activities and properties to achieve faster and more accurate amplification of the DNA templates. The enzyme mix permits efficient amplification of up to 20 kb from lambda DNA and up to 15 kb from genomic DNA.
Although the size of the DNA is generally not a critical factor, amplification is improved if genomic DNA is digested with a rarely cutting restriction enzyme (e.g., Not I). Target sequences are amplified slightly less efficiently when they are carried in closed circular form rather than in linear form. It is therefore preferable to linearize plasmid DNAs before they are used as templates in PCR.
Unit definition

One unit of the enzyme mix will incorporate 10 nmol of dNTPs into an acid-insoluble form in 30 min at 72°C under standard assay conditions using a DNA template.

Reaction buffer
B4 (x10, with Mg2+ )
Store at -20°C.
long-distance PCR.

Quality control

activity, SDS-PAGE purity, absence of endo- and exonucleases, specific performance tests.


1. Barnes, W.M. (1994) Proc. Natl. Acad. Sci. USA 91, 2216-2220. 2. Cheng, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 5695-5699.

Catalog #





Pack size

500 U

1000 U





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