- ThermoKlenS™-Thermostable DNA polymerase optimized for sequencing.
- Description
- ThermoKlenS™ (EC 2.7.7.7) is a modified Thermus aquaticus DNA polymerase lacking 5´-3´ exonuclease activity, which has improved capability to incorporate the nucleotide analogs (e.g. ddNTPs). The enzyme , when used in conjunction with thermostable inorganic pyrophosphatase (10:1), generates sequencing data of exceptional accuracy. Read lengths of more than 600 bases are routinely observed.
- Unit definition
One unit of the enzyme will incorporate 10 nmol of dNTPs into an acid-insoluble form in 30 min at 72°C under standard assay conditions using a DNA template.
- Reaction buffer
- B5 (x10, with Mg2+ ): 670 mM Tris-HCl, pH 9.1, 166 mM
(NH4)2SO4 , 35 mM MgCl2, 0.15% BSA.
- Storage
- Store at -20°C.
- Applications
- cycle sequencing.
Quality control
activity, SDS-PAGE purity, tested in cycle sequencing.
Reference
1. Tabor, S., Richardson, C.C. (1995) Proc. Natl. Acad. Sci. USA 92, 6339-6343. 2. Van der Horn, P.B. et al. (1997) Biotechniques 22, 758-762, 764-65.
- Catalog #
- T7.3
T7.4
Pack size
500 U
1000 U