ThermolasePR - High-fidelity DNA polymerase with proofreading activity.
ThermolasePR (EC is a thermostable DNA-dependent DNA polymerase cloned from the archaea Thermococcus litoralis and overexpressed in recombinant E.coli strain. The enzyme has 5´-3´ polymerase, 5´-3´ exonuclease (strand displacement) as well as 3´-5´ exonuclease (”proofreading“) activities. The fidelity of ThermolasePR DNA polymerse is 10-fold greater as that of Taq DNA polymerase (error rate is 4.5-5.7x10-5). The enzyme is extremely thermostable (half-life at 95°C 6.7 h, temperature optimum is 70-75°C). Up to 15 kb can be amplified from lambda DNA and up to 10 kb from genomic DNA.
Unit definition

One unit of the enzyme will incorporate 10 nmol of dNTPs into an acid-insoluble form in 30 min at 72°C under standard assay conditions using a DNA template.

Reaction buffer
B1 (x10, with Mg2+ ): 670 mM Tris-HCl, pH 8.8, 166 mM (NH4)2SO4, 15 mM MgCl2, 0.1% Tween 20.
Store at -20°C.
high-fidelity PCR; long template and accurate PCR.

Quality control

activity, SDS-PAGE purity, specific performance tests.


1. Mattila, P. et al. (1991) Nucleic Acids Res. 19, 4967-4973. 2. Kong, H.M. et al. (1993) J. Biol. Chem 268, 1965-1975. 3. Perler, F. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 5577. 4. Belkin, S., Jannasch, H.W. (1985) Arch. Microbiol. 141, 181-186.

Catalog #





Pack size

250 U

500 U





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