M.B. Enzymes GmbH

Antibiotics # Y3-Y6.

Description

Y3 Ampicillin (D(-)-a-Aminobenzylpenicillin, C₁₆H₁₈N₃NaO₄S, M.W.= 371,39) is a half-synthetic penicillin (b-lactam antibiotic), known to interfere with the peptidoglycan cross-linking in grown bacteria and to inhibit cell wall biosynthesis. Acts at pH 5.5-6.0. Stock solution is prepared at conc. of 50 mg/ml in water; the working concentration is 20-50 µg/ml.

Y4 Kanamycin A (Kanamycin sulfate, C₁₈H₃₆N₄O₁₁ x H₂SO₄, M.W.=582,58) binds to 70S ribosomal subunit, inhibits translocation and elicits miscoding. Stock solution is prepared at conc. of 10 mg/ml in water; the working concentration is 10-50 µg/ml. The solution is stable in pH range 6-8 at RT for 6-8 month (loss of activity approx. 1-2%); autoclaving at 120°C for 1 hour results in 5% loss of activity.
Y5 Tetracycline hydrochloride (C₂₂H₂₄N₂O₈xHCl, M.W.=480,9) inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to ribosome. Do not use medium with magnesium for selection of bacteria resistant to Tc because Mg²⁺ ions are antagonists of tetracycline. Stock solution is prepared at conc. of 10 mg/ml in 70% ethanol or in water; the working concentration is 10-50 µg/ml. Use freshly prepared stock solution; discard after 4 weeks of storage.
Y6 G418 (Geneticin, C₂₀H₄₀N₄O₁₀ x H₂SO₄, M.W.=692,70) is an aminoglycosidic antibiotic toxic to both pro- and eukaryotic cells. It acts by interfering with protein synthesis and is used for selection and maintenance of eucaryotic cells stably transfected with neomycin resistance gene. Stock solution is prepared at conc. of 40 mg/ml in PBS or water. The working solutions are 400 µg/ml for selection and 200 µg/ml for maintenance.

Storage

Store lyophilized powders at RT or at +4°C (G418), solutions at -20°C. Protect tetracycline from day light.

Catalog #

Y3.1

Y4.1
Y5.1 Y6.1

Pack size

5 g

5 g
5 g 1 g

Antibiotics # Y3-Y6.
Description	Y3 Ampicillin (D(-)-a-Aminobenzylpenicillin, C₁₆H₁₈N₃NaO₄S, M.W.= 371,39) is a half-synthetic penicillin (b-lactam antibiotic), known to interfere with the peptidoglycan cross-linking in grown bacteria and to inhibit cell wall biosynthesis. Acts at pH 5.5-6.0. Stock solution is prepared at conc. of 50 mg/ml in water; the working concentration is 20-50 µg/ml. Y4 Kanamycin A (Kanamycin sulfate, C₁₈H₃₆N₄O₁₁ x H₂SO₄, M.W.=582,58) binds to 70S ribosomal subunit, inhibits translocation and elicits miscoding. Stock solution is prepared at conc. of 10 mg/ml in water; the working concentration is 10-50 µg/ml. The solution is stable in pH range 6-8 at RT for 6-8 month (loss of activity approx. 1-2%); autoclaving at 120°C for 1 hour results in 5% loss of activity. Y5 Tetracycline hydrochloride (C₂₂H₂₄N₂O₈xHCl, M.W.=480,9) inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to ribosome. Do not use medium with magnesium for selection of bacteria resistant to Tc because Mg²⁺ ions are antagonists of tetracycline. Stock solution is prepared at conc. of 10 mg/ml in 70% ethanol or in water; the working concentration is 10-50 µg/ml. Use freshly prepared stock solution; discard after 4 weeks of storage. Y6 G418 (Geneticin, C₂₀H₄₀N₄O₁₀ x H₂SO₄, M.W.=692,70) is an aminoglycosidic antibiotic toxic to both pro- and eukaryotic cells. It acts by interfering with protein synthesis and is used for selection and maintenance of eucaryotic cells stably transfected with neomycin resistance gene. Stock solution is prepared at conc. of 40 mg/ml in PBS or water. The working solutions are 400 µg/ml for selection and 200 µg/ml for maintenance.
Storage	Store lyophilized powders at RT or at +4°C (G418), solutions at -20°C. Protect tetracycline from day light.
Catalog #	Y3.1	Y4.1	Y5.1	Y6.1
Pack size	5 g	5 g	5 g	1 g